Part:BBa_K763001:Experience

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• Shadowburn

Our team 2019 GDSYZX run the enzyme digestion verification and sequence test of plasmid GroE-AsRed2, then test the fluorescence intensity under different heat-induce time. (set gradient between 0 and 30 minutes.) We place the shaken fugus solution into 18℃,28℃,37℃, 42℃, and 55℃ pots for 10 minutes and cultivate the solution in static under room temperature. We test the OD600 value and Florence intensity 0 min, 30 min, 60 min, 90min, 120min,150min, 180min after the heat shock transformation. (measure condition: wavelength of excited light: 576nm, wavelength of emitting light: 592nm, E.coli competent :DH5α, chloramphenicol LB culture medium , Endonuclease XbaI and SpeI: enzyme test of GroE-AsRed2 plasmid.) The result indicates that the fluorescence intensity is the highest under 55 ℃ and that the fluorescence intensity all tends to reach their maximum at about 125 minutes after heat shock transformation under different temperature.

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